Short-term changes in intracellular ROS localisation after the silver nanoparticles exposure depending on particle size

نویسندگان

  • Akira Onodera
  • Fumiko Nishiumi
  • Kisa Kakiguchi
  • Atsushi Tanaka
  • Nami Tanabe
  • Aki Honma
  • Katsutoshi Yayama
  • Yasuo Yoshioka
  • Kumiko Nakahira
  • Shigenobu Yonemura
  • Itaru Yanagihara
  • Yasuo Tsutsumi
  • Yuichi Kawai
چکیده

Silver nanoparticles (AgNPs) induce the production of reactive oxygen species (ROS) and apoptosis. These effects are enhanced by smaller particles. Using live-cell imaging, we show that AgNPs induced ROS production rapidly in a size-dependent manner after exposure of cells to 70-nm and 1-nm AgNPs (AgNPs-70, AgNPs-1), but not AgNO3. Exposure of cells to 5 μg/mL each of AgNPs-70, AgNPs-1 or AgNO3 for 1 h decreased the cell viability by approximately 40%, 100% and 20%, respectively. ROS were rapidly induced after 5 and 60 min by AgNPs-1 and AgNPs-70, respectively, whereas AgNO3 had no detectable effect. ROS production detected using the reporter dichlorodihydrofluorescein was observed in whole cells and mitochondria 5 and 60 min after exposure to AgNPs-1. The present study is the first, to our knowledge, to report the temporal expression and intracellular localisation of ROS induced by AgNPs.

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عنوان ژورنال:

دوره 2  شماره 

صفحات  -

تاریخ انتشار 2015